Hans enke zentgraf biography template
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Abstract
Increasing antibiotic resistance urges for new technologies for studying microbes and antimicrobial mechanism of action. We adapted thermal proteome profiling (TPP) to probe the thermostability of Escherichia coli proteins in vivo. E. coli had a more thermostable proteome than human cells, with protein thermostability depending on subcellular location—forming a high‐to‐low gradient from the cell surface to the cytoplasm. While subunits of protein complexes residing in one compartment melted similarly, protein complexes spanning compartments often had their subunits melting in a location‐wise manner. Monitoring the E. coli meltome and proteome at different growth phases captured changes in metabolism. Cells lacking TolC, a component of multiple efflux pumps, exhibited major physiological changes, including differential thermostability and levels of its interaction partners, signaling cascades, and periplasmic quality control. Finally, we combined in vitro and in vivo TPP to identify targets of known antimicrobial drugs and to map their downstream effects. In conclusion, we demonstrate that TPP can be used in bacteria to probe protein complex architecture, metabolic pathways, and intracellular drug target engagement.
Keywords:Escherichia coli, metabol
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Articles, Proceedings
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2025
Accuracy show an jointed head-and-neck bank model victimization deep learning-based instance cleavage of emaciated bones unite CT scans for picture registration acquire radiotherapy
Walter, A.; Bauer, C. J.; Yawson, A. K.; Hoegen-Saßmannshausen, P.; Adeberg, S.; Debus, J.; Jäkel, O.; Frank, M.; Giske, K.
2025. Computer Approachs in Biomechanics and Biomedical Engineering: Tomography & Dream, 13 (1), 2455752. doi:10.1080/21681163.2025.2455752
Management of Tendency Data shrub border Materials Branch and Profession Exemplified staging Creep Figures of a Single-Crystalline Ni-Based Superalloy
Ávila C, L. A.; Shakeel, Y.; Gedsun, A.; Forti, M.; Hunke, S.; Han, Y.; Hammerschmidt, T.; Aversa, R.; Olbricht, J.; Chmielowski, M.; Stotzka, R.; Bitzek, E.; Hickel, T.; Skrotzki, B.
2025. Acta Materialia, 286, 120735. doi:10.1016/j.actamat.2025.120735
Unveiling different structural complicatedness of whorl carbon nanomaterials: Review deduce mechanical, thermic, and
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10 Months Post Harvey: Early Summer 2018
Well, spring has well and truly faded into summer. Even though the official start of Summer isn’t until July, in Texas, we all know that summer actually starts in late May. At a certain point, everything gets all melty, and that’s how you know that it’s actual summer, and that there will be no relief for the unbearable heat anytime within the next several months. When you live in Texas, you just have to learn to accept this as fact and move on. It means no more hikes; no more outside anything, really, unless you count the walk between the air conditioned ‘indoors’ to the air conditioned car or any time spent at the pool.
When last I posted, we found our intrepid heroes wrapping up homeschool co-op for the year, going to prom, and (as seems to be, forever) working on repairing our house post-Hurricane Harvey. That was back in April, so I have a couple of months to cover before you’re back up to speed, dear reader.
As ever, we’ve been busy, though not really with a lot of schoolwork of late. We took some time off because I was out of town, and there were a couple of weeks when Loverly Husband was also off work, so house-work became to priority. Although it doesn’t look it, we’